Journal: Science Advances

Article Title: Knockdown of deleterious miRNA in progenitor cell–derived small extracellular vesicles enhances tissue repair in myocardial infarction

doi: 10.1126/sciadv.abo4616

Figure Lengend Snippet: ( A ) Immunohistochemical staining of ischemic hearts to detect CD63 + total macrophages and iNOS + CD63 + M1-type macrophages (CD63 for red and iNOS for green staining). Scale bars, 100 μm. ( B ) Quantification of the total number of CD63 + macrophages. *** P < 0.001 and **** P < 0.0001. ( C ) Quantification of the ratio of iNOS − CD63 + cells (M2 type) to iNOS + CD63 + cells (M1 type) ( n = 8). ** P < 0.01 versus M2 macrophage proportion in the PBS group. ( D ) Blood circulating CD45 − CD90 + cell population (MSC) in total PBMCs from cardiac IR rats. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Twenty million PBMCs were incubated with allophycocyanin (APC)–conjugated anti-rat CD45 antibody (Miltenyi Biotec, North Rhine-Westphalia, Germany) and fluorescein isothiocyanate (FITC)–conjugated anti-rat CD90 antibody (Abcam, Cambridge, UK).

Techniques: Immunohistochemical staining, Staining

Journal: Journal of Translational Medicine

Article Title: Hypoxic BMSCs accelerate femur fracture healing via the METTL3/SLC2A3 m6A-glycolysis axis

doi: 10.1186/s12967-025-07510-2

Figure Lengend Snippet: Hypoxia induces higher osteogenic capacity in BMSCs . ( A ) Flow cytometry assesses the expression levels of BMSCs markers (CD29 and CD44) and another cell marker (CD45). ( B ) CCK-8 assay was conducted to assess the cell viability of BMSCs after stimulation with different concentrations of CoCl₂ for 72 h. * P < 0.05, ** P < 0.01, *** P < 0.001, one-way ANOVA. ( C ) Alizarin Red staining detects the osteogenic capacity. ( D ) Immunofluorescence assay showed the expression level of HIF1α. ( E ) ELISA analysis of ALP levels in Ctrl and Hypoxia groups. ( F ) Runx2, OCN, and Osx biomarkers were assessed using WB. *** P < 0.001, Student’s t -test

Article Snippet: BMSC identity was confirmed by flow cytometry (NovoCyte, Agilent, USA) using antibodies against CD29 (No. 130-119-165), CD44 (No. 130-107-802), and CD45 (No. 130-128-256; 1:100 diluted, Miltenyi Biotech, Germany).

Techniques: Flow Cytometry, Expressing, Marker, CCK-8 Assay, Staining, Immunofluorescence, Enzyme-linked Immunosorbent Assay

Journal: Journal of Diabetes Research

Article Title: Renal Protection by Genetic Deletion of the Atypical Chemokine Receptor ACKR2 in Diabetic OVE Mice

doi: 10.1155/2016/5362506

Figure Lengend Snippet: Knockout of the ACKR2 gene reduces leukocyte infiltration in diabetic mice. (a) Representative images of CD45 staining, original magnification 200x. (b) Quantitative analysis of leukocyte infiltration scored as CD45 positive pixel area per visual field. Twenty-four random fields from 3 mice per group were measured. ∗ indicates p < 0.05 versus OVE. Statistical comparisons were performed by one-way ANOVA.

Article Snippet: Renal inflammatory cell infiltration was evaluated by staining sections with rat anti-mouse CD45 antibody (Angio-Proteomie, Boston, MA).

Techniques: Knock-Out, Staining